Publication Details
Isomura, I.,
Palmer, S.,
Grumont, R. J.,
Bunting, K.,
Hoyne, G. F.,
Wilkinson, N.,
Banerjee, A.,
Proietto, A.,
Gugasyan, R.,
Wu, L.,
McNally, A.,
Steptoe, R. J.,
Thomas, R.,
Shannon, M. F.,
&
Gerondakis, S.
(2009).
c-Rel is required for the development of thymic Foxp3 CD4 regulatory T cells.
Journal of Experimental Medicine, 206 (13), 3001-3014.
https://doi.org/10.1084/jem.20091411
Abstract
During thymopoiesis, a unique program of gene expression promotes the development of CD4 regulatory T (T reg) cells. Although Foxp3 maintains a pattern of gene expression necessary for T reg cell function, other transcription factors are emerging as important determinants of T reg cell development. We show that the NF-κB transcription factor c-Rel is highly expressed in thymic T reg cells and that in c-rel−/− mice, thymic T reg cell numbers are markedly reduced as a result of a T cell–intrinsic defect that is manifest during thymocyte development. Although c-Rel is not essential for TGF-β conversion of peripheral CD4+CD25− T cells into CD4+Foxp3+ cells, it is required for optimal homeostatic expansion of peripheral T reg cells. Despite a lower number of peripheral T reg cells in c-rel−/− mice, the residual peripheral c-rel−/− T reg cells express normal levels of Foxp3, display a pattern of cell surface markers and gene expression similar to those of wild-type T reg cells, and effectively suppress effector T cell function in culture and in vivo. Collectively, our results indicate that c-Rel is important for both the thymic development and peripheral homeostatic proliferation of T reg cells.
Keywords
Peer-reviewed, T cells, CD4, regulatory T cells, Foxp3, c-Rel
